BRaf pAb

21271

Size：100 μl Price：$ 219 Brand：NewEast Place of Origin：USA Immunogen：

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BRaf Polyclonal Antibody

Cat. #: 21271

Gene Symbol: B-RAF, B-RAF1, RAFB1

Description: Anti-BRaf Rabbit Polyclonal Antibody

Background: B-Raf is a member of the Raf family of Ser/Thr protein kinases. It functions downstream of Ras to regulate the MAP kinase signaling pathway. Mutations in the B-RAF gene cause diseases. Inherited mutations in B-RAF cause cardiofaciocutaneous syndrome. Acquired mutations in B-RAF have been found in cancers.

Immunogen: Purified B-Raf protein, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions:
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:200

Concentration: 0.1mg/ml

Host Species: Rabbit

Format: Liquid

Clonality: Polyclonal

Isotype:

Purity: Purified from serum

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes B-Raf of vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry:

Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue with anti- B-Raf polyclonal antibody (Cat. #21271). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.

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BRaf pAb

IDH2(R172G)

IDH2(R172W)

IDH2(R172K)

IDH2(R172S)

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