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EYA2 mAb

26052

Size:100 μl Price:$ 239 Brand:NewEast Place of Origin:USA Immunogen:

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EYA2 Monoclonal Antibody

Cat. #:  26052
Gene Symbol:  EYA2
Description:   Anti-EYA2 Mouse Monoclonal Antibody
Background:   EYA2 (eyes absent homolog 2) functions as a tyrosine phosphatase that specifically dephosphorylates Tyr-142 of histone H2AX. EYA2 promotes efficient DNA repair and coactivates SIX1. Together with SIX1 and DACH2, EYA2 seems to be involved in myogenesis.
Immunogen:   A synthetic peptide near the N-terminus of human EYA2.
Applications:   ELISA, WB, IHC, IP
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:100-1:1000
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Anti-EYA2 antibody recognizes EYA2 of vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing.
Western blot:  

Western blot analysis of recombinant full-length EYA2 protein. Purified His-tagged EYA2 protein (Cat. # 10195) was blotted with anti EYA2 monoclonal antibody (Cat. # 26052).
Immunohistochemistry:  

Immunohistochemical analysis of paraffin-embedded human adenocarcinoma tissue with anti EYA2 monoclonal antibody (Cat. # 26052). Tissue section was fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as the secondary antibody.
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