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Albumin mAb

26090

Size:100 μl Price:$ 239 Brand:NewEast Place of Origin:USA Immunogen:

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Albumin Monoclonal Antibody

Cat. #:  26090
Gene Symbol:  ALB
Description:    Anti-Albumin Mouse Monoclonal Antibody
Background:    Albumin belongs to the ALB/AFP/VDB family. It comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones, and also plays a role in stabilizing extracellular fluid volume. Defects in Albumin will cause familial dysalbuminemic hyperthyroxinemia (FDH).
Immunogen:   Albumin protein, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:1000-1:5000
IHC: 1:50-1:100
Concentration:    1 mg/ml
Host Species:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   IgG
Purity:   Purified from ascites
Preservative: No
Constituents:   PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:   Anti-Albumin antibody recognizes Albumin from vertebrates.
Storage Conditions:  Store at -20°C. Avoid repeated freezing and thawing.

Western blot:  

Western blot analysis of Albumin(HSA) and BSA proteins.

Purified Albumin protein (lane 2) were blotted with anti Albumin monoclonal antibody (Cat. # 26090). BSA protein was loaded as a control (lane 1).
Immunohistochemistry:  

Immunohistochemical analysis of paraffin-embedded liver cancer tissue with anti Albumin monoclonal antibody (Cat. #26090). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as secondary antibody.

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