RAS(A146P)

26478

Size：100 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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RAS(A146P)

Cat. #: 26478

Gene Symbol: H-Ras; K-Ras; N-Ras

Description: Anti-RAS(A146P) Mouse Monoclonal Antibody

Background: The A146P mutation of KRas results in an amino acid substitution at position 146, from an alanine to a threonine. KRAS encodes a protein that is a member of the small GTPase superfamily. Ras A146P mutation results in decreased GTPase activity and constitutive signaling. It can be found in many tumors, such as lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.

Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of A146P, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions: ELISA: 1:1000-1:5000 IF: 1:50-1:100 IHC: 1:50-1:100

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes A146P mutant, but not wild type RAS of vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Immunofluorescence:

Immunofluorescence of cells expressing KRas proteins with anti-KRAS(A146P) antibody. HEK293T cells were transfected with pCDNA3-GFP-KRAS(A146P) plasmid, pCDNA3-GFP-KRas (WT) plasmid, pCDNA3-GFP-KRas (A146V) plasmid or pCDNA3-GFP-KRas (A146T) plasmid, then fixed and stained with anti-KRAS(A146P) monoclonal antibody (Cat. #26478).

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RAS(A146P)

IDH2(R172G)

IDH2(R172W)

IDH2(R172K)

IDH2(R172S)

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