RAS(G12S)

26186

Size：100 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

Add to cart

Download

Datasheet

MSDS

Product Description Reference citations(0) Related Products

RAS(G12S)

Cat. #: 26186

Gene Symbol: H-Ras; K-Ras; N-Ras

Description: Anti-RAS(G12S) Mouse Monoclonal Antibody

Background: Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G12S mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.

Immunogen: A synthetic peptide from the internal region of RAS which includes the mutation of G12S, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes G12S mutant, but not wild type RAS of vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Western blot:

Western blot analysis of recombinant RAS(G12S) and wild type proteins. Purified His-tagged RAS(G12S) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-RAS(G12S) monoclonal antibody (Cat. #26186). Immunofluorescence:

Immunofluorescence of cells expressing RAS proteins with Anti-RAS(G12S) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(G12S) plasmid, pCDNA3-GFP-Ras(WT)plasmid,pCDNA3-GFP-Ras(G12S) plasmid, pCDNA3-GFP-Ras(G12V)plasmid,pCDNA3-GFP-Ras(G12C)plasmid, pCDNA3-GFP-Ras(G12R)plasmid or pCDNA3-GFP-Ras(G12A)plasmid, then fixed and stained with Anti-RAS(G12S) monoclonal antibody (Cat. #26186).

Reference citations

No data available

Product Targets

Product Categories

RAS(G12S)

IDH2(R172G)

IDH2(R172W)

IDH2(R172K)

IDH2(R172S)

Contant Us