Email Us：info@neweastbio.com
Call Us：(+1) 610-945-2007
Email Us：info@neweastbio.com
Call Us：(+1) 610-945-2007
Size：100 μl
Price：$ 495
Brand：NewEast 
Place of Origin：USA
Immunogen：
| Cat. #: 26215 |
| Size: 100 µL |
| Gene Symbol: PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha |
| Description: Anti-PIK3CA(D549N) Mouse Monoclonal Antibody |
| Background: Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer. |
| Immunogen: A synthetic peptide from the internal region of PIK3CA which includes the mutation of D549N, human origin. |
| Applications: ELISA, WB, IHC |
| Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IHC: 1:50-1:100 |
| Concentration: 0.5 mg/ml |
| Host Species: Mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgG |
| Purity: Purified from ascites |
| Preservative: No |
| Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| Species Reactivity: Recognizes D549N mutant, but not wild type PIK3CA of vertebrates. |
| Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing |
| Western blot:
Western blot analysis of recombinant PIK3CA(D549N) and wild type proteins. Purified His-tagged PIK3CA(D549N) protein (lane 2) and corresponding wild type protein (lane 1) were blotted with Anti-PIK3CA(D549N) monoclonal antibody (Cat. #26215). |
Immunofluorescence: 
Immunofluorescence of cells expressing PIK3CA proteins with Anti-PIK3CA(D549N) antibody. HEK293T cells were transfected with pCDNA3-GFP-PIK3CA (WT) plasmid (left column) or pCDNA3-GFP-PIK3CA(D549N) plasmid (right column), then fixed and stained with Anti-PIK3CA(D549N) monoclonal antibody (Cat. #26215). |
Select By Alphabet
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
Subscribe to our latest email
(+1) 610-945-2007 info@neweastbio.com sale@neweastbio.com 840 First Avenue, Suite 400, King of Prussia, PA 19406
Copyright © 2010 - 2024 NewEast Biosciences | All rights reserved
Bioactive Transmembrane Proteins Antibodies for Transmembrane Proteins G Protein | GTPase
Add to cart
Buy now
Download
