RheB(Y35N)

26338

Size：100 μl Price：$ 495 Brand：NewEast Place of Origin：USA Immunogen：

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RheB(Y35N)

Cat. #: 26338

Size: 100 µL

Gene Symbol: RHEB

Description: Anti-RheB(Y35N) Mouse Monoclonal Antibody

Background: RheB (Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras-family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, RheB is widely expressed in other tissues and may be induced by growth factor stimulation. RheB has an important role in regulating the insulin/TOR signaling pathway. The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting RheB via tuberin's GAP activity.

Immunogen: A synthetic peptide from the internal region of RHEB which includes the mutation of Y35N, human origin.

Applications: ELISA, WB, IHC

Recommended Dilutions: ELISA: 1:1000-1:2000 WB: 1:500-1:1000 IHC: 1:50-1:100

Concentration: 1 mg/ml

Host Species: Mouse

Format: Liquid

Clonality: Monoclonal

Isotype: IgG

Purity: Purified from ascites

Preservative: No

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Species Reactivity: Recognizes Y35N mutant, but not wild type RHEB of vertebrates.

Storage Conditions: Store at -20°C. Avoid repeated freezing and thawing

Western blot:

Western blot analysis of recombinant RHEB(Y35N) and wild type proteins. Purified His-tagged RHEB(Y35N) protein (lane2) and corresponding wild type protein (lane1) were blotted with Anti-RheB(Y35N) monoclonal antibody (Cat. #26338).

Immunofluorescence:

Immunofluorescence of cells expressing RHEB proteins with Anti-RHEB(Y35N) antibody. HEK293T cells were transfected with pCDNA3-GFP-RHEB (WT) plasmid (left column) or pCDNA3-GFP-RHEB(Y35N) plasmid (right column), then fixed and stained with Anti-RheB(Y35N) monoclonal antibody (Cat. #26338).

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