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PDL1 mAb (Atezolizumab)

28018

Size:100 μg Price:$ 199 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   28018
Product Name:   Anti-PDL1 (Atezolizumab) Monoclonal Antibody
Synonyms:   PD-L1;CD274;B7-H1;PDCD1L1;PDCD1LG1
Description:    Anti-PDL1 (atezolizumab ) Monoclonal Antibody
Background:    Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
Applications:    ELISA; Flow Cyt
Recommended Dilutions:    ELISA 1:5000-10000; Flow Cyt 1:100
Host Species:    Humanized
Isotype:    IgG1
Purification:    Purified from cell culture supernatant by affinity chromatography
Species Reactivity:    Human PDL1
Constituents:    Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human PDL1, mFc-His tagged protein 11151 can bind Anti-PDL1 Neutralizing antibody in a linear range of 0.24-7.81 ng/ml.

Figure 2. HEK293 cell line transfected with irrelevant protein  (B) and human PDL1  (A) were surface stained with anti-PDL1 neutralizing antibody 1µg/ml (atezolizumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.
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