CD138 (DM45) Rabbit mAb

28336

Size：100 μg Price：$ 445 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: 28336

Product Name: Anti-CD138(DM45) Rabbit Monoclonal Antibody

Synonyms: SDC1; Syndecan-1; CD138; SYND1; SDC

Description: Anti-CD138 antibody(DM45) Rabbit Monoclonal Antibody

Background: Syndecan-1 (SYND1 or SDC1) is also known as CD antigen CD138; is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding; cell signaling; and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 : SDC1 protein functions as an integral membrane protein and participates in cell proliferation; cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a useful marker for plasma cells; but only if the cells tested are already known to be derived from blood.

Applications: ELISA; Flow Cyt

Recommended Dilutions: ELISA 1:5000-10000; Flow Cyt 1:100

Host Species: Rabbit

Isotype: Rabbit IgG

Purification: Purified from cell culture supernatant by affinity chromatography

Species Reactivity: Human CD138

Constituents: Lyophilized from sterile PBS, pH 7.4. 5 % – 8% trehalose is added as protectants before lyophilization.

Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).

Figure 1. Expi 293 cell line transfected with irrelevant protein (A) and human CD138 (B) were surface stained with Rabbit anti-CD138 monoclonal antibody 1µg/ml ( clone: DM45) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.	Figure 2. Flow cytometry data of serially titrated Rabbit anti-CD138 monoclonal antibody (clone: DM45) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 3. Affinity ranking of different Rabbit anti-CD138 mAb clones by titration of different concentration onto H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

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