CD276 Rabbit pAb

221696

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: S221696

Product Name: Anti-CD276 Rabbit Polyclonal Antibody

Synonyms: B7H3; B7-H3; B7RP-2; 4Ig-B7-H3

UNIPROT ID: Q5ZPR3 (Gene Accession - NP_001019907 )

Background: The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen: Synthetic peptide of human CD276

Applications: ELISA, WB, IHC

Recommended Dilutions: IHC: 25-100;WB: 200-1000;ELISA: 5000-10000

Host Species: Rabbit

Clonality: Rabbit Polyclonal

Isotype: Immunogen-specific rabbit IgG

Purification: Antigen affinity purification

Species Reactivity: Human, Mouse, Rat

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol

Research Areas: Cancer, Immunology, Stem Cells

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 221696(CD276 Antibody) at a dilution of 1/20(Cytoplasm and Cell membrane).	In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 221696(Anti-CD276 Antibody) at dilution 1/20.
Gel: 8%SDS-PAGE, Lysate: 40 μg; Lane 1-4: PC-3, 231, LNCAP and 293T cell lysates; Primary antibody: 221696(CD276 Antibody) at dilution 1/350; Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution; Exposure time: 15 seconds

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CD276 Rabbit pAb

TUBB3 Rabbit pAb

GAPDH Rabbit pAb

TUBA4A Rabbit pAb

ALB Rabbit pAb

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