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NEK9 Rabbit mAb

262592

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N262592
Product Name:   Anti-NEK9 Rabbit Monoclonal Antibody
Synonyms:   NC; APUG; NERCC; LCCS10; NERCC1
UNIPROT ID:   Q8TD19
Background:    Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.
Immunogen:    A synthetic peptide of human NEK9
Applications:    WB,IHC-F,IHC-P,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R03-1F1
MW:    Calculated MW: 107 kDa; Observed MW: 107 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of NEK9 in Hela lysates using NEK9 antibody

Immunocytochemistry analysis of NEK9 (green) in HT-1080 using NEK9 antibody,and DAPI(blue).

Western blot analysis of NEK9 in rat skeletal muscle lysates using NEK9 antibody.

Immunohistochemistry analysis of paraffin-embedded Human colon cancer using NEK9 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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