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Phospho-p27 Kip 1 (Ser10) Rabbit mAb

262651

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N262651
Product Name:   Anti-Phospho-p27 Kip 1 (Ser10) Rabbit Monoclonal Antibody
Synonyms:   CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1
UNIPROT ID:   P46527
Background:    The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
Immunogen:    A synthetic phosphopeptide corresponding to residues surrounding Ser10 of human p27 KIP 1
Applications:    WB,IHC-P,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R05-4F1
MW:    Calculated MW: 22 kDa; Observed MW: 27 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using p27 KIP 1 (Phospho-Ser10) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Phospho-p27 KIP 1 (Ser10) in Hela, 3T3 lysates using Phospho-p27 Kip 1 (Ser10) antibody.

Western blot analysis of Phospho-p27 KIP 1 (Ser10) in C6 lysates using Phospho-p27 KIP 1 (Ser10) antibody.
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