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SQSTM1/p62 Rabbit mAb

262933

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N262933
Product Name:   Anti-SQSTM1/p62 Rabbit Monoclonal Antibody
Synonyms:   p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
UNIPROT ID:   Q13501
Background:    Autophagy receptor that interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1.
Immunogen:    Recombinant protein of human SQSTM1
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R06-9J2
MW:    Calculated MW: 48 kDa; Observed MW: 62 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    AutophagyyyyyyAutophagosome
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using SQSTM1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunocytochemistry analysis of SQSTM1/p62 (green) in U87-MG using SQSTM1/p62 antibody,and DAPI(blue).

Western blot analysis of SQSTM1 in K562, 3T3, Hela lysates using SQSTM1 antibody.
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