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Phospho-Src (Tyr530) Rabbit mAb

263198

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N263198
Product Name:   Anti-Phospho-Src (Tyr530) Rabbit Monoclonal Antibody
Synonyms:   SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src
UNIPROT ID:   P12931
Background:    Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).
Immunogen:    A synthetic phosphopeptide corresponding to residues surrounding Tyr529 of human Src
Applications:    WB,IP
Recommended Dilutions:    WB: 1/500-1/1000 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R03-8H8
MW:    Calculated MW: 60 kDa; Observed MW: 60 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Rat
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of Src (Phospho-Tyr529) in CHO-K1 lysates using Src (Phospho-Tyr529) antibody.

Western blot analysis of Phospho-Src (Tyr529) in rat lung lysates using Phospho-Src (Tyr530) antibody.
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