BUBR1 Rabbit mAb

263242

Size：100 μl Price：$ 349 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: N263242

Product Name: Anti-BUBR1 Rabbit Monoclonal Antibody

Synonyms: BUB1B; BUBR1; MAD3L; SSK1; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1

UNIPROT ID: O60566

Background: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE.

Immunogen: A synthetic peptide of human BubR1

Applications: WB,ICC/IF,IP

Recommended Dilutions: WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20

Host Species: Rabbit

Clonality: Rabbit Monoclonal

Clone ID: R01-4C1

MW: Calculated MW: 120 kDa; Observed MW: 120 kDa

Isotype: IgG

Purification: Affinity Purified

Species Reactivity: Human

Conjugation: Unconjugated

Modification: Unmodified

Constituents: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Research Areas: Cell Biology

Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing

Immunocytochemistry analysis of BubR1 (green) in hela using BubR1 antibody,and DAPI(blue)

Western blot analysis of BubR1 in Hela, A549, MCF-7, U251 lysates using BUBR1 antibody.

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