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SF3B1 Rabbit mAb

263546

Size:100 μl Price:$ 349 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:   N263546
Product Name:   Anti-SF3B1 Rabbit Monoclonal Antibody
Synonyms:   SF3B1; SAP155; Splicing factor 3B subunit 1; Pre-mRNA-splicing factor SF3b 155 kDa subunit; SF3b155; Spliceosome-associated protein 155; SAP 155
UNIPROT ID:   O75533
Background:    SF3B1 is subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA.
Immunogen:    A synthetic peptide of human SF3B1
Applications:    WB,IHC-F,IHC-P,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R08-6I2
MW:    Calculated MW: 146 kDa; Observed MW: 155 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Hamster,Rat
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of SF3B1 in Hela, CHO-K1, C6, Ramos lysates using SF3B1 antibody

Immunocytochemistry analysis of SF3B1 (green) in 293T using SF3B1 antibody,and DAPI(blue).

Western blot analysis of SF3B1 in 3T3 lysates using SF3B1 antibody.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using SF3B1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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