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Human PROM1 Full Length Protein

11027

Size:50 μg Price:$ 4400 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:     11027
Product Name:   Human PROM1 Full Length Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   AC133; CD133; CORD12; MCDR2; MSTP061; PROML1; RP41; STGD4
Target:    PROM1
UNIPROT ID:    O43490
Description:    Human PROM1 full length protein-synthetic nanodisc
Background:     A pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
Species/Host:    HEK293
Molecular Weight:    The human full length PROM1 protein has a MW of 97.2 kDa
Molecular Characterization:    The human full length PROM1 protein has a MW of 97.2 kDa
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure1. Elisa plates were pre-coated with Flag Tag PROM1-Nanodisc (0.2 µg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with PROM1-Nanodisc is 5.105ng/ml.

Figure2. Human PROM1-Nanodisc, Flag Tag on SDS-PAGE
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