Human EGFR Protein, His Tag

11218

Size：100 μg Price：$ 479 Brand：NewEast Place of Origin：USA Immunogen：

Add to cart

Download

Datasheet

MSDS

Product Description Reference citations(0) Related Products

Cat.#: 11218

Product Name: Human EGFR Protein

Size : 10 µg, 50 µg and 100 µg

Synonyms: EGFR;RBB;RBB1;ER1;IG61;ENA

Target: EGFR

UNIPROT ID: P00533

Description: Recombinant human EGFR Protein with C-terminal 6xHis tag

Background: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.

Species/Host: HEK293

Molecular Weight: The protein has a predicted molecular mass of 69.4 kDa after removal of the signal peptide.The apparent molecular mass of EGFR-His is approximately 100-130 kDa due to glycosylation.

Molecular Characterization: EGFR(Leu25-Ser645) 6×His tag

Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.

Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human EGFR Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human EGFR, His tagged protein (11218) can bind Anti-EGFR Antibody 28043 in a linear range of 0.64-80.0 ng/ml.

Reference citations

No data available

Product Targets

Product Categories

Human EGFR Protein, His Tag

Human CLDN6 Full Length Protein

Human CB1 Full Length Protein

Human CCR4 Full Length Protein

Human OR2H1 Full Length Protein

Contant Us