Human AXL Protein, His Tag

11193

Size：100 μg Price：$ 479 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: 11193

Product Name: Human AXL Protein

Size : 10 µg, 50 µg and 100 µg

Synonyms: AXL;FO

Target: AXL

UNIPROT ID: P30530

Description: Recombinant human AXL protein with C-terminal 6xHis tag

Background: The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6 (Gas6). This gene may be involved in several cellular functions including growth, migration, aggregation and anti-inflammation in multiple cell types. Alternative splicing results in multiple transcript variants of this gene.

Species/Host: HEK293

Molecular Weight: The protein has a predicted molecular mass of 46.6 kDa after removal of the signal peptide.

Molecular Characterization: AXL(Ala26-Pro449) 6×His tag

Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.

Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human AXL Protein, His Tag on SDS-PAGE under reducing condition.	Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human AXL, His tagged protein (11193) can bind Anti-AXL Antibody 28042 in a linear range of 0.64-80.0 ng/ml.
Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human AXL, His tagged protein (11193) can bind Human NTD, mFc Tagged protein 11298 in a linear range of 0.006-0.16 µg/ml.	Figure 4. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human AXL, His tagged protein (11193) can bind Human NTD, hFc Tagged protein 11299 in a linear range of 0.0012-0.16 µg/ml.

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Human AXL Protein, His Tag

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