Human MICA Protein, His Tag

11263

Size：100 μg Price：$ 525 Brand：NewEast Place of Origin：USA Immunogen：

Add to cart

Download

Datasheet

MSDS

Product Description Reference citations(0) Related Products

Cat.#: 11263

Product Name: Human MICA Protein

Size : 10 µg, 50 µg and 100 µg

Synonyms: MIC-A;PERB11.1

Target: MICA

UNIPROT ID: Q29983

Description: Recombinant Human MICA Protein with C-terminal 6xHis tag

Background: This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Species/Host: HEK293

Molecular Weight: The protein has a predicted molecular mass of 33.5 kDa after removal of the signal peptide. The apparent molecular mass of MICA-His is approximately 55-70 kDa due to glycosylation.

Molecular Characterization: MICA (Glu24-Trp307) 6×His tag

Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.

Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human MICA Protein, His Tag on SDS-PAGE under reducing condition.	Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human MICA, His tagged protein (11263) can bind Human NKG2D, mFc tagged protein (11200) in a linear range of 0.064-1.6 µg/ml.
Figure 3. Flow cytometry analysis with 15 µg/ml Human MICA Protein, His Tag (11263) on Expi293 cells transfected with Human NKG2D protein and Human DAP10 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Reference citations

No data available

Product Targets

Product Categories

Human MICA Protein, His Tag

Human CLDN6 Full Length Protein

Human CB1 Full Length Protein

Human CCR4 Full Length Protein

Human OR2H1 Full Length Protein

Contant Us