Human HVEM Protein, His Tag

11253

Size：100 μg Price：$ 555 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: 11253

Product Name: Human HVEM Protein

Size : 10 µg, 50 µg and 100 µg

Synonyms: ATAR;CD270;HVEA;HVEM;LIGHTR;TR2

Target: HVEM

UNIPROT ID: Q92956

Description: Recombinant Human HVEM Protein with C-terminal 6xHis tag

Background: This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants.

Species/Host: HEK293

Molecular Weight: The protein has a predicted molecular mass of 18.2 kDa after removal of the signal peptide.The apparent molecular mass of HVEM-His is approximately 25-55 kDa due to glycosylation.

Molecular Characterization: HVEM(Leu39-Val202) 6×His tag

Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.

Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human HVEM Protein, His Tag on SDS-PAGE under reducing condition.	Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human HVEM, His tagged protein (11253) can bind Human BTLA,mFc-His tagged protein 11174 in a linear range of 0.64-80 ng/ml.
Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human HVEM, His tagged protein (11253) can bind Human CD160,hFc tagged protein 11179 in a linear range of 1.28-160 ng/ml.	Figure 4. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human HVEM, His tagged protein (11253) can bind Human LIGHT, hFc tagged protein 11175 in a linear range of 0.32-40 ng/ml.
Figure 6. Flow cytometry analysis with 15 µg/ml Human HVEM Protein, His tag (11253) on Expi293 cells transfected with human LIGHT (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).	Figure 6. Flow cytometry analysis with 15 µg/ml Human HVEM Protein, His tag (11253) on Expi293 cells transfected with human LIGHT (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

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Human HVEM Protein, His Tag

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