Human CD30 Protein, His tag

11291

Size：100 μg Price：$ 555 Brand：NewEast Place of Origin：USA Immunogen：

Add to cart

Download

Datasheet

MSDS

Product Description Reference citations(0) Related Products

Cat.#: 11291

Product Name: Human CD30 Protein

Size : 10 µg, 50 µg and 100 µg

Synonyms: TNFRSF8;D30;1S166E;i-1

Target: CD30

UNIPROT ID: P28908

Description: Recombinant human CD30 protein with C-terminal 6xHis tag

Background: The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Species/Host: HEK293

Molecular Weight: The protein has a predicted molecular mass of 39.3 kDa after removal of the signal peptide. The apparent molecular mass of CD30-His is approximately 55-70 kDa due to glycosylation.

Molecular Characterization: CD30(Phe19-Lys379) 6×His tag

Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.

Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human CD30 Protein, His Tag on SDS-PAGE under reducing condition.	Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD30, His tagged protein (11291) can bind Anti-CD30 Neutralizing antibody 28026 in a linear range of 0.13-80.0 ng/ml.
Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD30, His tagged protein (11291) can bind Human CD30 Ligand,mFc-His tagged protein 11167 in a linear range of 2.56-320 ng/ml.	Figure 4. Flow cytometry analysis with 1 µg/ml Human CD30 Protein, His tag (11291) on Expi293 cells transfected with human CD30L (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Reference citations

No data available

Product Targets

Product Categories

Human CD30 Protein, His tag

Human CLDN6 Full Length Protein

Human CB1 Full Length Protein

Human CCR4 Full Length Protein

Human OR2H1 Full Length Protein

Contant Us