Human LAG3 Protein, His Tag

11315

Size：100 μg Price：$ 479 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: 11315

Product Name: Human LAG3 Protein

Size : 10 µg, 50 µg and 100 µg

Synonyms: LAG-3;CD223

Target: LAG3

UNIPROT ID: P18627

Description: Recombinant Human LAG3 Protein with C-terminal 6xHis tag

Background: Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]

Species/Host: HEK293

Molecular Weight: The protein has a predicted molecular mass of 47.0 kDa after removal of the signal peptide. The apparent molecular mass of LAG3-His is approximately 55-70 kDa due to glycosylation.

Molecular Characterization: LAG3(Leu23-Leu245) 6×His tag

Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.

Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human LAG3 Protein, His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 1 µg/ml (100 µl/well) Human LAG3 , His tagged protein (11315) can bind Anti-LAG3 Neutralizing antibody 28069 in a linear range of 16-80ng/ml.

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Human LAG3 Protein, His Tag

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