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Human EPHA5 Protein, His Tag

11411

Size:100 μg Price:$ 415 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:     11411
Product Name:   Human EPHA5 Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   CEK7;EHK-1;EHK1;EK7;HEK7;TYRO4
Target:    EPHA5
UNIPROT ID:    P54756
Description:    Recombinant human EPHA5 protein with C-terminal 6xHis tag
Background:     This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Aug 2013]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 61.6 kDa after removal of the signal peptide. The apparent molecular mass of EPHA5-His is approximately 70-100 kDa due to glycosylation.
Molecular Characterization:    EPHA5(Pro25-Pro573) 6×His tag
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human EPHA5 Protein, His Tag on SDS-PAGE under reducing condition.
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