Human LIF Protein, hFc Tag

11532

Size：100 μg Price：$ 389 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: 11532

Product Name: Human LIF Protein

Size : 10 µg, 50 µg and 100 µg

Synonyms: Leukemia inhibitory factor; factor;MLPLI;mfilermin

Target: LIF

UNIPROT ID: P15018

Description: Recombinant human LIF protein with C-terminal human Fc tag

Background: The protein encoded by this gene is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Species/Host: HEK293

Molecular Weight: The protein has a predicted molecular mass of 46 kDa after removal of the signal peptide. The apparent molecular mass of LIF-hFc is approximately 55-70 kDa due to glycosylation.

Molecular Characterization: LIF(Ser23-Phe202) hFc(Glu99-Ala330)

Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.

Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human LIF Protein, hFc Tag on SDS-PAGE under reducing condition.

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Human LIF Protein, hFc Tag

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