Human EGF Protein, hFc Tag

11683

Size：100 μg Price：$ 550 Brand：NewEast Place of Origin：USA Immunogen：

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Cat.#: 11683

Product Name: Human EGF Protein

Size : 10 µg, 50 µg and 100 µg

Synonyms: Urogastrone

Target: EGF

UNIPROT ID: P01133

Description: Recombinant human EGF protein with N-terminal human Fc tag

Background: This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Species/Host: HEK293

Molecular Weight: The protein has a predicted molecular mass of 32.4 kDa after removal of the signal peptide. The apparent molecular mass of hFc-EGF is approximately 35-45 kDa due to glycosylation.

Molecular Characterization: hFc(Glu99-Ala330) EGF( Asn971- Arg1023)

Purity: The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Formulation & Reconstitution: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.

Storage & Shipping: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human EGF Protein, hFc Tag on SDS-PAGE under reducing condition.

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Human EGF Protein, hFc Tag

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