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Human APLN Protein

12281

Size:100 μg Price:$ 415 Brand:NewEast Place of Origin:USA Immunogen:

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Cat.#:     12281
Product Name:   Human APLN Protein
Size :    10 µg, 50 µg and 100 µg
Synonyms:   APEL;XNPEP2
Target:    APLN
UNIPROT ID:    Q9ULZ1
Description:    Recombinant human APLN Protein with C-terminal human Fc tag
Background:     This gene encodes a peptide that functions as an endogenous ligand for the G-protein coupled apelin receptor. The encoded preproprotein is proteolytically processed into biologically active C-terminal peptide fragments. These peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1. [provided by RefSeq, Feb 2016]
Species/Host:    HEK293
Molecular Weight:    The protein has a predicted molecular mass of 32.3 kDa after removal of the signal peptide. The apparent molecular mass of APLN-hFc is approximately 25-55 kDa due to glycosylation.
Molecular Characterization:    APLN(Gly23-Phe77) hFc(Glu99-Ala330)
Purity:    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Formulation & Reconstitution:    Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
Storage & Shipping:    Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Figure 1. Human APLN Protein, hFc Tag on SDS-PAGE under reducing condition.
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